全文获取类型
收费全文 | 11420篇 |
免费 | 682篇 |
国内免费 | 1001篇 |
专业分类
林业 | 924篇 |
农学 | 510篇 |
基础科学 | 544篇 |
1220篇 | |
综合类 | 5566篇 |
农作物 | 862篇 |
水产渔业 | 569篇 |
畜牧兽医 | 1574篇 |
园艺 | 887篇 |
植物保护 | 447篇 |
出版年
2024年 | 37篇 |
2023年 | 219篇 |
2022年 | 497篇 |
2021年 | 468篇 |
2020年 | 434篇 |
2019年 | 427篇 |
2018年 | 275篇 |
2017年 | 514篇 |
2016年 | 341篇 |
2015年 | 476篇 |
2014年 | 531篇 |
2013年 | 704篇 |
2012年 | 988篇 |
2011年 | 1026篇 |
2010年 | 995篇 |
2009年 | 885篇 |
2008年 | 897篇 |
2007年 | 852篇 |
2006年 | 659篇 |
2005年 | 589篇 |
2004年 | 355篇 |
2003年 | 201篇 |
2002年 | 231篇 |
2001年 | 187篇 |
2000年 | 195篇 |
1999年 | 52篇 |
1998年 | 9篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1987年 | 2篇 |
1981年 | 2篇 |
1962年 | 2篇 |
1956年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 234 毫秒
51.
AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis. 相似文献
52.
Numerical studies on transient heat transfer characteristics of air-array-jet impingement with a small jet-to-plate distance and a large temperature difference between nozzles and plate were presented.The dimensionless jet-to-plate distance(H/D)was 0.2,and non-dimensional nozzle-to-nozzle spacing(S/D)was 3,4,5 and 6,respectively.It is found that the quenching time is shortened at a constant total mass flow at air jet inlet m·(m·=218.21 kg/h),and the heat transfer uniformity is deterio-rated as S/D increases.However,the adding reversed-flow nozzles can shorten the quenching time of the glass plate considerably with a modest change in the heat transfer uniformity.The results at variable m·are the same as those at a fixed m·.Furthermore,the parity and arrangement of nozzles are also discussed,It is found that an odd number of nozzles is more beneficial for transient heat transfer.Based on these results,an appropriate proposal for ultra-thin glass tempering process is presented. 相似文献
53.
54.
为研究关中冬小麦植株蒸腾和土壤蒸发规律,利用2 a冬小麦小区控水试验实测数据,率定和验证了双作物系数SIMDual_Kc模型在关中地区的适用性.用大型称重式蒸渗仪的实测蒸散量值(或水量平衡法计算值)与模型模拟值进行对比.结果表明:SIMDualKc模型可较准确地模拟关中不同水分条件下冬小麦蒸散量,且模拟精度较高.模型估算的平均绝对误差为0.643 3 mm/d.模型估算的冬小麦初期、中期和后期的基础作物系数分别为0.35,1.30,0.20.另外,模型还可以较准确地估算不同水分供应条件下的土壤水分胁迫系数、土壤蒸发量和植株蒸散量.冬小麦整个生育期,土壤蒸发主要发生在作物生育前期,中期较低,后期略微增大;植株蒸腾主要发生在作物快速生长期和生长中期,整个生育期中呈先增大后减小的趋势. 相似文献
55.
AIM: To explore the clinical significance of Krüpple-like factor 15 (KLF15) protein expression in the patients with lung adenocarcinoma for exploring the therapeutic and prognositic biomarkers of lung cancer. METHODS: Four cases of lung adenocarcinoma tissues and matched adjacent tissues were collected from our hospital, and the expression of KLF15 protein in these tissues was analyzed by Western blot. At the same time, 72 cases of archived paraffin-embedded samples and clinical data of the patients with lung adenocarcinoma were also collected. The KLF15 protein expression in the archived paraffin-embedded lung adenocarcinoma samples was detected by immunohistochemical staining. The correlations between KLF15 protein expression and clinical characteristics of the patients including prognosis were also analyzed. In addition, the KLF15 protein was up-regulated in A549 cells, and then the effects of KLF15 protein on the viability of the cells were measured by CCK-8 assay. RESULTS: The protein expression of KLF15 in the 4 cases of lung adenocarcinoma tissues was significantly lower than that in matched paracancerous tissues. Fifty-three cases of lung adenocarcinoma specimens showed low expression or no expression of KLF15 protein in total 72 cases (73.6%). The 5-year survival rate of the patients with high expression of KLF15 protein in their specimens was higher than that of the patients with the low expression of KLF15 protein (P<0.01), and the expression of KLF15 protein was significantly correlated with the pathological staging (P<0.01) and T stage (P<0.01) of the patients with lung adenocarcinoma. Furthermore, the low expression of KLF15 protein was an important poor prognostic indicator of the patients. Up-regulation of KLF15 protein in the A549 cells significantly inhibited the growth of the cells. CONCLUSION: KLF15 inhibits the growth of lung adenocarcinoma cells. It could be used as a therapeutic target and a prognostic biomarker for the patients with lung adenocarcinoma. 相似文献
56.
公共绿地作为开放空间,公众可以共享自然环境及社会资源。而当下公共绿地中不同群体的参与度存在较大差异,尤其是年轻群体参与度低。笔者以上海浦东新区部分公共绿地中年轻群体活动参与度为研究对象,从年轻群体的低参与度、活动意愿及管理者角度等方面,分析了年轻群体低参与度的原因,认为原因主要是年轻群体缺乏业余时间、绿地可达距离较远、活动内容缺乏等。在调查基础上,从活动载体、信息渠道、服务方式等方面提出建议,主张公共绿地管理者转变服务理念、开拓有益活动、建立信息平台、优化空间资源,以提高社会服务效能。 相似文献
57.
58.
北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量(P=0.028)、体高(P=0.017)和体长(P=0.037)具有显著影响,SBS02对体质量(P=0.033)和体高(P=0.034)具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。 相似文献
59.
在对广东省主要珍贵树种上的线虫进行调查时从格木Erythrophleum fordii上分离到一种隐皮孢囊线虫,经形态特征观察和测量数据分析,将其鉴定为卡勒萨隐皮孢囊线虫Cryphodera kalesari。其诊断特征为:雌虫椭圆形至近球形,头部具有1个唇盘和2~3个唇环,口针长33.6~37.8μm,阴门唇突出,阴门与肛门之间区域凹陷,肛阴距为42~72μm;2龄幼虫头部具1个明显的唇盘和3个唇后环纹,口针长24.8~29.5μm,口针基部球前缘凹陷,侧区3条侧线,尾长圆锥形,长38~53.5μm,尾末端细圆,透明尾长17.1~25.1μm,侧尾腺孔位于肛门后2~5环;雄虫未发现。本研究首次获得了卡勒萨隐皮孢囊线虫的rDNA(LSU D2D3和ITS)序列,为此线虫的鉴定提供了可靠的分子数据。本研究还分析了卡勒萨隐皮孢囊线虫与本属其他种类的系统进化关系。卡勒萨隐皮孢囊线虫为中国的地理新记录种。格木为隐皮孢囊线虫的新寄主。 相似文献
60.
WU Xue-lei CAI Yao-wu ZHUANG Zhi-zhong CHEN Yuan-jing GUO Ren-jie ZHENG Mao-song 《园艺学报》2015,31(12):2169-2175
AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression. 相似文献